Journal: bioRxiv

Article Title: The interferon-inducible GTPase MxB promotes capsid disassembly and genome release of herpesviruses

doi: 10.1101/2022.01.25.477704

Figure Lengend Snippet: Unbiased hierarchical clustered heat map showing the log 2 fold changes of IFN-induced proteins (GO type-I IFN) identified from capsids-host protein sediments from cytosol of resting Mφ, or IFN-induced Mφ IFN macrophages. For each protein, the fold change was calculated based on their abundance (iBAQs) in V 1 , V 0.5 and V 0.1 capsids as compared to their abundance in D capsids, using a linear scale from violet being the lowest to dark green being the highest. (*) and (**) design the proteins with an FDR corrected p-value < 0.05 and < 0.01, respectively.

Article Snippet: The cells were cultured with 1000 U/mL human type I IFN-α2a (Mφ IFN ; R&D Systems, Minneapolis, Minnesota, USA) or left untreated for 16 h. A549 cells were cultured in DMEM with 10% FCS.

Techniques:

Journal: PLoS Biology

Article Title: Epigenetic Reprogramming of the Type III Interferon Response Potentiates Antiviral Activity and Suppresses Tumor Growth

doi: 10.1371/journal.pbio.1001758

Figure Lengend Snippet: (A) Gel mobility shift assay was performed using the wild-type (WT) probe (the −434∼−401 region of the IFNLR1 promoter) and the mutant (MUT) probe ( CCAAT motif substituted with AACCG ), incubated with U87 cell nuclear extracts, poly (dA∶dT), 100-fold excess of cold probe, and the indicated antibodies. (B) ChIP analysis was performed on the IFNLR1 promoter in Huh7 and U87 cells with NF-YC and control IgG antibodies. (C–D) NF-YA and IFNLR1 expression was examined by RT-qPCR in Huh7 cells transfected with scrambled or NF-YA-specific siRNAs. (E, G) Expression of NF-YA and receptor subunits of type I, II, and III IFNs was examined by RT-qPCR in Huh7 cells stably expressing scrambled or NF-YA-specific shRNAs. (F) Lysates from Huh7 stable cell lines were harvested and used for WB using the indicated antibodies. Lamin B serves as loading control for nuclear proteins. (H–I) NF-YA and IFNLR1 expression was measured by RT-qPCR in PHHs infected with lentiviruses encoding scrambled or NF-YA-specific shRNAs. In all panels, data represent the mean and SEM of at least three experiments.

Article Snippet: CD4 + T cells were cultured in complete RPMI 1640 medium supplemented with 100 U/ml IL-2 and 100 ng/ml IL-7 and stimulated with 10 µg/ml PHA every 72 h. Cells were treated with recombinant human IFN-α2a, IFN-β, human IFN-λ1, IFN-λ2, IFN-λ3 (R&D Systems), murine IFN-λ2 (PeproTech), or universal type I IFN (hIFN-αA/D) (PBL Interferon Source).

Techniques: Mobility Shift, Mutagenesis, Incubation, Expressing, Quantitative RT-PCR, Transfection, Stable Transfection, Infection